Culture And Values Of New Zealand Essay - 1548 Words

FORMAL MEETING GUIDE TWO CULTURES This Report is about the multi culture of New Zealand SAHIL KUMAR 14075027b Submitted to ANIKA VATS Assignment no: 11647 Contents INTRODUCTION 3 MAORI CULTURE 3 OTHER CULTURE 3 ABOUT HUI 3 PROCESS OF MEETING 4 AGENDA 4 DATE AND TIME 4 BEHAVIOUR AND FORMAL 4 ROLES 4 PREPARE IN ADVANCE 5 COMMUNICATION SKILL 5 NOTICE OF MEETING 5 REGULATION OF MEETING 5 RECORD OF THE MEETING 5 COUNTDOWN 5 AGENDA 6 PLANNING AND PROCEDURES FOR MEETING 6 GLOSSARY 6 Reference 8 INTRODUCTION The Purpose of this report is to understand the culture and values of New Zealand culture in terms of their living condition, behaviour, society, aspect, ethics, rules, regulations, greetings, treating, food and environment. I will be focusing on the main cultures of New Zealand as it is a multi cultural society. MAORI CULTURE Maoris lifestyle is interesting society of New Zealand. By nature they are welcoming and reverence the other society. They respect the people by shaking handshake and smile or make legitimate acquaintance. They have their own particular routine administration for treating the visitors. On the off chance that they are dealing with a tribal social event they welcomed visitors in no place styles. The customary capacity will continues going 2 or 3 hours. As an issue of first criticalness, they invited visitors in their routine social affair house. Visitors need to oblige them as social affair and talkShow MoreRelatedCultural Values And Cultural Value Orientations1439 Words   |  6 Pagesimmersed in our own culture, it can be challenging to grasp the difference in cultural pattern taxonomies orientations of other cultures. Cultural pattern taxonomies has been defined in the textbook, Communication between Cultures, â€Å"as the dominant beliefs and values of a culture.â €  Cultural value orientation varies among cultures and ultimately affects the way we communicate interpersonally. 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Meanwhile, based on my ethno-cultural identity, my philosophy of nursing would be defined as that caringRead MoreUsing Cultural Theories Analyzing the Main Issue of the New Zealand Office of atT1674 Words   |  7 Pages pp87-88) organizational culture is a common assumption, value or norm system among members of an organization which is based on their behaviors to help organization to achieve the abilities they need to adapt itself to the environment, create uniformity and internal integrity. This essay will analyze the main issue of ATT’s New Zealand office by using the cultural theories and the reflections after the analyz ing. In the case of ATT GIS, ATT’s office in New Zealand by Thomas, David C (2003Read MoreEssay On Zealand Business1101 Words   |  5 PagesNEW ZEALAND BUSINESS COMMUNICATIONS INTRODUCTION Understanding cultural variances in the world of business, is extremely crucial in developing valuable relationships in a global market for your company. 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Overall, China ranks as very different from New Zealand in regards to values, beliefs, norms andRead MoreCorporate Co Branding : The Case Of Adidas And The All Blacks1527 Words   |  7 Pagesare not only used to identify and differentiate products. Nowadays, brands identify services, organisations, art, ideas, people, places, etc. Corporate branding is the concept of the marketing efforts undergone to represent a corporation’s system of values; it is the expression of the organisation’s identity. A well-established corporate brand has the ability to create close relationships with stakeholders and can assist the organisation in achieving its goals. Co-branding can be defined as a â€Å"formRead MoreHai Di Lao Case Study1703 Words   |  7 PagesHRM strategy and then evaluate the lessons that New Zealand organisations can gain from an analysis of this case study. Within this evaluation, this essay will address the role of HRM strategy in creating competitive advantage and the applicability of such strategy to New Zealand firms. Ultimately, this essay argues that the case study of Hai Di Lao provides valuable insight as to how to achieve resource-based competitive advantage however New Zealand organisations ought to exercise caution in seekingRead MoreEducation Is Important For A Nation s Most Populous City With A Wide Demographic Of Multicultural Individuals Essay1621 Words   |  7 Pagesperception and engagement with Waitangi Day. Within the wider context of New Zealand, Auckland is the nation’s most populous city with a wide demographic of multicultural individuals. With significant external immigration to the city, future growth is to be expected. Therefore, it is important for the topic of Waitangi education to be investigated, to ensure that systems are put in place for future pupils that will go through the New Zealand schooling system, to ensure that they are accurately taught about

Poor Working Conditions in the Age of Industrialization

The age of industrialization brought many changes to American society. Industrialization had a huge impact in the development of the nineteenth century. Industrialization was important because it led to new technologies and inventions that tremendously improved every day life. The technical innovations and the inventions of railroads brought people into the cities. Due to this, industries grew and more jobs were available. The need for workers put women and children into the workforce, providing cheap labor, and resulting in poor working conditions. The texts â€Å"Modern America is Born† by Milton Meltzer, â€Å"Robber Barons and Rebels† by Howard Zinn, and â€Å"Hostile Employers See Yourselves as Others Know You† by Samuel Gompers, talks about poor working conditions that happened during industrialization. The working conditions in the late 1800s and the early 1900s were very poor. In 1869 the completion of the first transcontinental railroad was a new develop ment in America. The railroad was built mostly by immigrants that would work for low wages. The employers that then worked on the railroad had certain issues. In â€Å"Modern America is Born† the author states, â€Å"For decades both freight and passenger trains suffered from two devices that kept service slow and dangerous [†¦] The brakeman had to stand between the cars so he could slide the link into the socket and drop the pin to hold the cars together [†¦] An even worse part of the job was the manual braking of the cars to slow or stopShow MoreRelated2002 Ap Euro Dbq: Manchester Essay1067 Words   |  5 PagesThe Effects of Industrialization on Manchester, England 1750-1850 England in the 18th and 19th centuries changed dramatically as a result of the Industrial Revolution, which had many effects on the social structure of England and increased the gap between the rich and the poor. 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Key individuals helped advance the process of a more industrialized America, however corruption in business and politics also monopolies in certain parts of industries came withRead MoreA Social Morality Of The Victorian Age1355 Words   |  6 PagesOscar Martin Professor Stephen Mendonca English 2323 2 August 2015 A Social Morality The Victorian age ranged from 1830 to 1901, during this time England reached its highest point as a world imperial power. Industrialization and the reign of Queen Victoria (1837 to 1901) played a major role in England’s success. The overwhelming industrialization caused a population boom that changed England’s population from two million to six million people. The abundance of people created new social problemsRead MoreIndustrialization During The Late Abraham Lincoln1052 Words   |  5 Pageschange and industrialization created a huge gap between the rich and the poor and made it hard to rise to the top. Industrialization changed America from an agricultural country to one based on manufacturing goods and services. This change created advancement and expansion for the nation as a whole. Industrialization was very helpful to the economy but was an impediment for the working class, like Sam Patch. In the book, Sam Patch by Paul E. Johnson, it is evident how industriali zation restrictedRead MoreThe Life Of Immigrants During The Gilded City Of New York954 Words   |  4 Pages The life of immigrants in the gilded city of New York. As the cities of New York grew rapidly through the advancement of industrialization and urbanization, the wealthier exploited the many immigrants settling in. Rapid urbanization made life for immigrants challenging because of the cheap and poor constructions of the tenements, and as well as overcrowding. 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During the Gilded Age, social, political, and economic reform movements wereRead More19th Century Industrialization Essay1485 Words   |  6 Pages19th Century Industrialization Nineteenth Century Industrialization During the second half of the nineteenth century, the United States experienced an urban revolution unparalleled in world history up to that point in time. As factories, mines, and mills sprouted out across the map, cities grew up around them. The late nineteenth century, declared an economist in 1889, was not only the age of cities, but the age of great cities. Between 1860 and 1910, the urban population grew from 6 millionRead MoreRole Of Women During The Nineteenth Century1456 Words   |  6 PagesAmerican economy in general, enjoyed the benefits of industrialization, many Americans were not so fortunate. Immigrants and former soldiers moved to the cities in search of jobs, money, and new opportunities. This created scarcity in employment and other resources. 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Federation of Automobile Dealers Associations Report Free Essays

|Federation of Automobile Dealers Associations | | | | | |   | |Home   | | | |Truck Freightage Defies Trends in Economy: IFTRT Report | |   | |Defying the buoyant data being released by various agencies about the expansion of economic activities and soaring corporate profits, | |the trucking business, consisting of 3 million trucks, has failed to look up in last 4 weeks. The truck freightage has remained flat | |on most of the trunk routes despite increase in cost of operation due to 2 diesel price hikes (Dec 31 and Dec 15†² 03) totaling Rs. 2/- | |per litre (10%) and have, in fact, sharply declined on trunk routes passing through Uttar Pradesh due to resumption of overloading by | |trucks. We will write a custom essay sample on Federation of Automobile Dealers Associations Report or any similar topic only for you Order Now | | |On the other hand various State Governments had withdrawn Gold token/ passes/ cards, which permitted over-loading of trucks in excess | |of permitted weight, in order to get release of their share of Central Road Fund withheld since April 2003. The Central Government has| |stopped Central Road Fund to those State Governments, which were not only permitting but sponsoring overloading of trucks in | |contravention of Central Motor Vehicle Act, 1988 by issuing Gold Cards/ passes/ tokens to truckers against fixed monthly/ quarterly | |fee. The Central Government has taken a firm view that â€Å"over-loading of the vehicles cause significant damage to road surface†¦ | | | |Recently, the State Government of Uttar Pradesh withdrew the Gold Card scheme w. e. f. Dec. 15, 03 and a month later Rajasthan did the | |same w. e. f. 1, Jan 04. The subsequent stringent enforcement of CMV Act, 1988 by U. P. Transport Department by not permitting the | |entry/passing through of overloaded t rucks from the State resulted in sharp increase in truck freightage by 4% – 7. 5% during the | |fortnight (Dec 1, – Dec 15, 03) and unsettled the trucking business in region. However, this anti-overloading drive was short-lived | |and has collapsed in the U. P. State, by and large. Now, overloaded trucks are plying and passing through the State merrily. Thus, | |truck freightage once again is being dictated by the over-loading of vehicles that existed before Dec. 1, 03. In the last four weeks | |the truck freightage for the trunk routes passing through U. P. has dropped by 3. 5% – 6. 8%, according to the monthly update released by| |Indian Foundation of Transport Training Research (IFTRT). | | | |Union Government outsmarted by Rajasthan | | | |Followed by U. P. the Rajasthan Government, too, had withdrawn its Gold card/token scheme from 1, Jan 04 to get the Central Road Fund | |released immediately. However, the State Government, very smartly has replaced the earlier special Gold Token Scheme with another | |†AMNESTY SCHEME† by charging multi slab fee to permit unhindered over-loading of trucks in excess of prescribed weight limit. The 1st | |Jan. ’04 notification has been â€Å"modified† to pacify the Central Government, which had again refused to release the money from Central | |Road Fund. But, plying of over loaded commercial vehicles continues under the patronage of State Transport Department, points out the | |report. |   | | | |TRUCK (16. 2 TON GVW) Hire charges/rates (Rs. per round trip 21 Dec’03 – 21 Jan’04 | |Route / Round Trip |Period |Truck Hire charges |Diesel Price increase Impact | |9 ton pay load | |change/Round Trip/Period |(per round trip) 31 Dec’03 | | | |21 Dec’03 – 21 Jan’04 | | | Hire Charges |Hire Charges | | | | |21 Dec’03 |21 Jan’04 | | | | | | |Rs. |% |Rs. |% | |Delhi – Mumbai – Delhi |28,800/- |28,800/- |No Change |NC |(+) 620/- |(+) 5 | |Delhi – Nagpur – Delhi |28,400/- |28,400/- |No Change |NC |(+) 610/- |(+) 5 | |Delhi – Kolkata – Delhi |34,700/- |33,000/- |(-) 1,700/- |(-) 5. 0 |(+) 630/- |(+) 5 | |Delhi – Guwahati – Delhi |69,400/- |67,000/- |(-) 2,400/- |(-) 3. |(+) 850/- |(+) 5 | |Delhi – Hyderabad – Delhi |43,100/- |43,100/- |No Change |NC |(+) 770/- |(+) 5 | |Delhi – Chennai – Delhi |59,000/- |59,000/- |No Change |NC |(+) 870/- |(+) 5 | |Delhi – Bangalore – Delhi |44,800/- |45,000/- |(+) 200/- |(+) 0. 5 |(+) 770/- |(+) 5 | |Delhi – Ranchi – Delhi |33,300/- |31,300/- |(-) 2,000/- |(-) 6. 8 |(+) 620/- |(+) 5 | |Delhi – Raipur – Delhi |29,300/- |29,300/- |No Change |NC |(+) 630/- |(+) 5 | |Delhi – Kandla – Delhi |18,300/- |18,300/- |No Change |NC |(+) 340/- |(+) 5 | |Delhi – Bilaspur – Delhi |30,300/- |30,300/- |No Change |NC |(+) 620/- |(+) 5 | How to cite Federation of Automobile Dealers Associations Report, Papers

Andrea Castle v. Sage Direct Marketing Pty Ltd.

Question: Describe about Andrea Castle who was an employee with Sage Direct Marketing Pty Ltd. Answer: Andrea Castle was an employee with Sage Direct Marketing Pty Ltd. She was employed under an Enterprise Agreement. The enterprise agreements are the collective agreements that are made between employers and employees about the terms of the conditions of employment (Freedland, et al 2016). The Fair works commission approve the process of the enterprise agreements that are made. They also assess the terms of the contractual agreement. She witnesses a robbery in action in a social gathering. She sent out an email to the employees to stay safe and that she saw a colored arm reaching for a purse. The use of the term colored arm and sending out a personal correspondence using the office email was the reasons for her being fired from the job. The email policy of the company states that the emails sent by the employees should be based only for the business purpose and the content of the email should not be offensive to others. The use of the colored arm description was deemed to be offensive by the managers. The company states that she had violated the internal email policy and the terms of the enterprise agreement. Andrea was to sue the company was wrongful termination. She cites that she had posted the email for the welfare of the people and posted information that was what she had witnessed during the course of the robbery. Owing to this the action of the company was deemed unfair by Andrea. This is the reason for he r to consider seeking unlawful termination. The purpose of this analysis is to analyze the validity of Andreas claim by using common law action and Fair Work Act 2009. In the initial section there will be discussion of the actual terms of the common law action and Fair Work Act 2009 Terms of common law action and Fair Work Act 2009 In Australia, the employees and the company are bound by a common law contract or modern aware or enterprise agreement along with National Employment standards (Poole, 2016). When discussing about the issues of the employment law it is important to first understand the structure of the employment law in Australia. Statute and Common law. Statute law is the law that is created by the parliament. Common law is the rules that has been determined by the judges. The courts follow the mandates and the precedence of the previous cases. From the perspective of implementation, the parliament takes precedence. The courts interpret the laws and also the ambiguities based on the specific terms of the case. Employment law operates in the basic employment relationship that involves common law contract that has been regulated by the statute. Fair Work Act 2009: Unfair dismissal Unfair dismissal is when the employees are dismissed from the job in a harsh or unreasonable manners. Fair Work would analyze the claim if it was harsh or unjust and is the case was not a case of genuine redundancy. They would also look into the grounds for dismissal of the employee. The clauses that are considered if a particular case was harsh or unjust is based on the reason given by the company. The Fair Work commission will analyze if the action of the employee and the conduct (Freedland et al., 2016). The people who can apply for unfair dismissal are the people who have worked for the minimum employment period of one year and the people who have been forced to resign from their jobs. The eligible employees should be covered by the terms of the registered agreement and have an annual earnings of more than $138900. The employees have to apply within 21 days. The company that dismisses the employee should also prove redundancy. The redundancy occurs when the employer shows that they no longer need the work done by the employee. The employee redundancy needs to be proved. The issue with the Fair Work Act is that the dismissal claim cannot be resolves between the parties. The factors for determination if a case was fair or unfair is not clear (Freedland et al., 2016). The factors that are considered by the Fair Work Act is if the reason for dismissal was valid, if the person was notified of the reason, if the person was given opportunity to respond to a particular allegation, unreasonable refusal by the employer to have a person present, the factors that contributed towards unsatisfactory performance and the degree in which the employee were affected by the action of the dismissed employee and the impact of the dismissal on the company. The employee reason given to respond will be analyzed. The employed person should have a support person during the course of the dismissal. Previous warning to the employee will be analyzed. The history of conduct of the employee would be in purview. This has been detailed in Section 387 (Freedland et al., 2016). The re medies are either reinstatement or compensation by the company. Again this is based on the facts of the individual case. The legislative minimum rights of the employee are governed by the enterprise agreement or the modern awards and the common law contract along with the implied employment term. Legislative minimum rights Certain right and entitlements are created by the parliament to protect all the employees. and their rights. For example, the right to leave is mandated by the National employment service which is a part of the Fair Work Act 2009. The Fair Work act also discusses about the right to belong in unions or protect employees against discrimination. Added to this the federal and the state laws mandates the ways in which it can provide remedies for unfair dismissal. Enterprise agreement/modern awards provide detailed protections and regulations based on the specific industries and occupations. Enterprise agreements in general are the business specific agreements that is made by the groups of employees and their employer. The enterprise agreement is a contractual agreement that is approved by the Fair Work commission. Common law contracts are the basic relationship that exists between the employer and the employee in a civil contract. In this the employee agrees to work for the employer for exchange of payment. The employment contract is similar to the civil contract. The terms of the employment contract are based on each subjective cases. But according to the law they need to follow the legislative minimum rights and the modern aware or enterprise agreement. The courts will uphold the contract when the parties have willingly agreed to the terms of the signed contract. Another tricky aspect to the courts is the implied terms of the contractual agreement (Susskind, 2013). Implied terms are the reasonable notice of termination between the employee. The implied duty of mutual trust and confidence is considered in this analysis. The implied duty of trust and confidence is that the employee should work in good faith efforts and not in the manner of termination. Modern companies need to be registered after the implementation of the Fair Work Act 2009. These statutory requirements regulate the rules regarding employment. The employee and employer relationship are bound by the common law contractual requirements. The enterprise agreement has precedence over the common law contractual terms. All these terms form a part of the agreement. Analysis of the case According to the Fair Work 2009 act, the company that choses to dismiss an employee should ensure that there is redundancy by the employee. But this was not the case. The employee was fired for sending one email. She was not disciplined rather was dismissed. In the case of Anderson v Thiess Pty Ltd[2014] FWC 6568, Mr. Anderson was summarily dismissed for sending a work email that was offensive to the people belonging to the Muslim faith. In spite of the case being offensive and against the policy of Thiess Anderson was awarded compensation (Legg, 2014) . The dismissal was considered to be harsh because of only the previous verbal warning given to Anderson. He was above the age of 65 and he would have difficulty obtaining other employment. The Thiess also did not give Anderson enough time to accept the contents of the email or apologize to the people for offending them (Legg, 2014) . Based on this case, it can be argued that Andrea was not given any form of warning by the company. Under the clause of the Fair Work she should have been given some place to voice her opinion instead of firing her (McKendrick, 2014). She was not given the opportunity to express or take back the email. The company did not find any issues with the work conduct of Andrea. It can be argued that the company based on the case of Anderson that they should have given her some other means of disciplining instead of dismissing her. The redundancy aspect was also not addressed by the company to prove that her firing would not cause the company any considerable losses. In Wise v Mildura Aboriginal Corporation, the applicant lodged a complaint for unfair dismissal. The employment was terminated after the employer did a police check that determined the applicant was charged with possession of ecstasy and unlawful possession of explosives in 2012. But the conduct in question was minor hence the courts let him go with a small fine of 350$ (Moran and Mallman, 2015). The courts explained that the dismissal were harsh and unjust. The reason given by the courts is that the conduct of the employee was not so serious to repudiate the damage of the company. Andrea can argue using this fact that there was no damage done to the reputation of the company by sending out the email. She had only merely stated the events to protect other employees. There was no indication that she wanted to act in malice against the colored people or anyone in particular (Southey and Southey, 2016). She can use this argument to cite the reason for unfair dismissal. She could argue t hat her conduct did not breach any violations or cause the company any reputable or tangible damage. In the case of OKeefe v The Good Guys (2011), Good Guy company fired OKeefe because he had made derogatory comments on Facebook page about one of his colleagues and the company in general. He was dismissed from the company owing to the actions. OKeefe filed an unfair dismissal but the Fair Work Act deemed the dismissal to be unreasonable because he had posted the particular comment outside working hours and not used any names in the post. It was considered by the courts that the actions of the employee were to demean the company and the reputation of the company (Wilson, 2016). Hence it was determined to be a fair ground for dismissal by the company. The company can argue that the comments about the colored hand were abusive to some of the employees and that would be perceived as discrimination by these employees. Similarly, in the case of Serafini v Holcim (Australia) Pty Ltd (2011), the truck driver posted traffic accidents while driving and posted the pictures on You tube and his social media page. Shortly after he was fired for posting pics during the work hours (Wilson, 2016). The Fair Work deemed the dismissal to be valid. Since he was not working according to the prescribed mandates and posting on social media instead. Using this case, the company can state that Andrea was not working during the working hours instead she was posting unrelated content that was not towards the business purpose of the company. Citing this they can say that she was not working the companys work during this time instead was sending out emails with agenda. Deductions from this analysis The courts usually consider the intent and the complete reason behind firing of the employees in each of these cases. It is difficult to understand the precedence of the courts as the courts have ruled in many ways in the past cases. Certain common factors can be understood in these cases. In these cases, the intent of the employees is considered by the courts. The look at the reason behind the employees making a particular action. In Andreas case this factors that would work in her favor are the fact that she wanted to help the employees, there was no malice intended, she did not cause the reputation of the company to be affected as she was only citing the evidence she had witnessed and most importantly she was not given any opportunity to explain a stance. These factors could be argued as the factors that contributed towards the unfair dismissal of the employees. The company also did not show the ways in which she was redundant or her action had caused the company any considerable loss. The company wanted to maintain an appearance owing to which she was fired. But she had signed the contract and agreed to follow the mandates of the email policy. The contract law usually is considered when there is a contract that is signed between willing adults. She was also using company official mail for purpose other than business during working hours. The contract also claimed that the terms are bound to change. The terms related to determination of what is right and unfair is not very clear in the implementation of the Fair work act by the cases. These causes obscurity in the ways of implementation of the Act (Stewart, 2012.). The most important factor that would aid in the case of Andrea was that she was not given an opportunity to present her case and her intent would be considered by the Fair Work Act while making the determination. Conclusion Andrea Castle was fired for sending out an email warning the employees to be careful from a colored hand. She wants to file a suit for unfair dismissal. Common contract law is the ultimate law that is considered in the cases of the employee employer relationship. Apart from this she had sent an email during working hours that was not related to the business aspects of the company. These factors could work against Andrea. However, the Fair Work Act 2009 was implemented to protect the rights of the employees. Andrea can argue that she was not given any space to voice her opinion or take back the email that she had sent to the employee. She did not have any support system when this dismissal occurred. Andrea dismissal was not proved under redundancy clause. There have been previous cases where the Fair Work has considered reinstatement of the employee or providing the employer with a certain penalty for firing under unfair clauses that was harsh. The courts look into the subjective natu re of the cases and make determination. It has been considered in this analysis after weighing in on the positives and the negatives of the case that Andrea has a strong case to present to the courts. Reference: Freedland, M., Bogg, A., Cabrelli, D., Collins, H., Countouris, N., Davies, A.C.L., Deakin, S. and Prassl, J. eds., 2016.The Contract of Employment. Oxford University Press. Legg, M., 2014. Class action settlements in Australia-the need for greater scrutiny.Melb. UL Rev.,38, p.590. McKendrick, E., 2014.Contract law: text, cases, and materials. Oxford University Press (UK). Moran, A. and Mallman, M., 2015. Understanding Social Cohesion in Shepparton and Mildura: Literature. Poole, J., 2016.Textbook on contract law. Oxford University Press. Southey, K. and Southey, K., 2016. To fight, sabotage or steal: are all forms of employee misbehaviour created equal?.International Journal of Manpower,37(6), pp.1067-1084. Stewart, A., 2012. Australian labour law in transition: The impact of the fair work act.New Zealand Journal of Employment Relations (Online),37(1), p.3. Susskind, A., 2013. Unfair dismissal benchbook.Law Society Journal: the official journal of the Law Society of New South Wales,51(11), p.24. Wilson, J., 2016. Employment law: A balancing act: Effective management of workplace disrepute by employees.Ethos: Official Publication of the Law Society of the Australian Capital Territory, (239), p.38.

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The eNotes Blog 8 Perfect Gift Ideas for BookLovers

8 Perfect Gift Ideas for BookLovers 1. Books on the Wall Its the holiday season- a perfect time to give your book-loving friends and family (or yourself, of course) a little something-something. We put together a curated list of our favorite bookish gifts with dorm life and personal style in mind. If we missed anything literarily amazing, be sure to let us know in the comments below so we can include it in part two. 1. Books on the Wall Books on the Wall are minimalist book posters that display readable text from your favorite works of classic literature, like  Romeo and Juliet,  Alice in Wonderland, and  Pride and Prejudice. 2. Library Card Pillows Missing the stamped library return slips of yesteryear? Us too!  These handmade pillows, brought to you by  dirtsa studio, are screen-printed by hand on cotton canvas, and both front and back look like a classic library checkout card. 3. Custom Book Necklaces Get a favorite book cover on a necklace and never be without it. Etsy seller Compliment can customize the perfect gift for you or the bookworm youre shopping for. 4. Shirts from Out of Print Out of Print has tons of unique designs celebrating our favorite stories. Bonus: Each purchase helps to fund literacy programs and book donations to communities in need while also supporting authors, publishers, and artists. Take all of our money. 5. Book Bags by Krukru Studios Etsy seller Krukru Studios employs professional in-house seamstresses to bring you bookish messenger bags. Covering titles like  The Hobbit,  Fantastic Beasts and Where to Find Them,  Lolita, and  The Bell Jar, these book bags are sure to be one of the most thoughtful (and thoughtfully made) gifts you could give. 6. Literary Board Games Board games are the gifts that keep on giving. Break the ice with newly made, nerdy friends for a night of bibliophilic fun. Peruse Bustles list of 11 literary board games and plan your next game night. 7. Bags by Book Lover Gifts These totes are totes the perfect bag to help you manage your library book haul. Book Lover Gifts also has quite the selection of novel-inspired stationary, accessories, posters, and more. 8. Mugs by Overdue Industries Literature lovers and coffee go together like lions, witches, and wardrobes, and coffee mugs are always a great gift. Overdue Industries has a solid selection of popular and cult bookish mugs  on Etsy. (We also all awwd to death at this Frog and Toad baby onesie.)

Weddings in The Middle East, Europe and America Research Paper

Weddings in The Middle East, Europe and America - Research Paper Example How the food is prepared and what they eat is significant to the ceremony. Furthermore, there are related religious ceremonies and meals that accompany wedding celebrations with concomitant health connotations. Therefore, this paper will compare weddings among Middle Easterners, Europeans and the Americans discussing their history, food symbolism, religious celebrations and health connotations. To begin with, the history of wedding in the United States is rather interesting. Traditionally, weddings were seen as a means of wealth and not necessarily out of desire or love. In fact, wedding symbolized the financial security that the groom provides to the bride’s family when the couple marries. Furthermore, brides were chosen in line with their economic worth. It precisely had nothing to do with affection. However, at the turn of the nineteenth century, couples began marrying for love (Wallace, 2004). In contrast to American wedding, Arab weddings were arranged marriages. When a young man reaches the appropriate for marriage, his family will go out and search for a potential bride. The process of investigation takes into account the girl’s physical beauty, cleanliness, education, educational level and her qualities as a house wife. Additionally, the groom’s family takes consideration of the behavior and reputation of the bride’s family (Monger, 2004 ). On the other hand, European wedding traditions are largely borrowed from other traditions. It is worthwhile noting that wedding traditions vary among European nations. Traditional European customs involved kidnapping bride and fighting off the bride’s tribesmen using a sword. Thereafter, the groom would hide the bride for one month in an unknown location; a process known as the ‘honeymoon’. However, things changed during the Victorian era. In the contemporary Europe, wedding borrows heavily from Christian practices. Notable is the white dress, denoting ‘white wedding’. The white dress

An Assessment of Training - Part II Assignment Example | Topics and Well Written Essays - 250 words

An Assessment of Training - Part II - Assignment Example But having said all these, I have a question or an issue with the selection made for the training program. The training program selected specific people belonging to mid-level management. My question therefore has to do with why not making provision to include at least a sample from all other management levels so that the competency gained could be evenly distributed across the organization. I was particularly happy that you identified the gap that exists in management practice when it comes to the measurement of outcomes and drivers associated with HR. It was therefore a step in the right direction that the proposed training program was made to be around this all important area of organizational development. My major issue with your training program however has to do with the limited use of assessment metrics and modules in knowing or determining the outcome with the program. As quote (Becker, Huselid & Ulrich, 2001) noted, the real measure of the success of a training program is in the extent to which the outcomes match the objectives. It would have therefore been important if you clearly stated your objectives and supported them with a metric that makes it possible for you to assess the training. Going into the future also, I strongly suggest that all training programs can be as thoroughly involving of all employees as

International Marketing - Theory Essay Example | Topics and Well Written Essays - 2500 words

International Marketing - Theory - Essay Example The purpose of laying importance of marketing and marketing plans in the first paragraph was to narrow down the topic towards marketing strategy, its formulation and its purposes for the firm it self. Now when we talk about strategy planning in marketing, we basically tend to make a match between organizational resources and its goals i.e. what an organization can really do by utilizing its resources and what its managers wanted to do (Kotabe 2007). So the strategy basically matches or ties up both ends of the same string so it could be implemented and formulated. If we delve deeper into the topic we see that marketing strategy formulation is of two types; one is based on the General marketing strategy and the other one is based on decision strategy. I have explained them in the next paragraphs to follow. The company I have chosen for this assignment is Dell Inc., and no body can deny its market share, value and prowess up to this date. I will start with the sector analysis and compa ny analysis and then will move on to the marketing strategies. Dell comes under information technology sector. ... The situation started to get better after 2001 and showed a great improvement after year 2003 when the average salary of a computer employee was more than double than what other people were getting from their respective professions. The industry labor force also increased from nil to 3.1% which broke the records in the US labor force industry as it was a growth of more than 75% as compared to previous records. Growth in the information technology industry is there because of the demand factors all around the world. The demand factors included the invention of nano-technology as more and more industries are heading towards this technology, emerging markets like India coming into the field with better and more IT products with better software programs, rise in demand of computers in the Asian markets, companies around the world are looking for cutting edge techs which is only possible if more and more sophisticated software and hardware are being made. Emerging markets in central and S outh America is also one of the reasons for the boom in this sector (Kerin 2008). Apart from this all, another reason for the boom in this sector is the mergers and acquisitions which took place after the dot com crisis. Those mergers and acquisitions compelled companies to pool in their resources and funnel up their energies so to invest in research and development and come up with new products which would give them a competitive edge (Srivastava 2008). This is also a very pertinent cause to explain and relate to the boom in the information technology sector. This rearrangement in the market made suppliers few in numbers which brought major giants on neck n neck competition with each other. This also lowered the switching costs

High Performance Liquid Chromatography (HPLC) 214

High Performance Liquid Chromatography (HPLC) 214 Introduction High performance liquid chromatography 214 is the most widely used of all of the analytical separation techniques. The reasons for the popularity of the method is its sensitivity, ready adaptability to accurate quantitative determinations, suitability for separating non-volatile species or thermally fragile ones, wide spread applicability to substance that are of prime interest to industry, many fields of science and the public. The applications of chromatography have grown explosively in the last fifty years owing not only to the development of several new types of chromatographic techniques but also to the growing need by scientist for better methods for characterizing complex mixtures. General methodology for the development of new HPLC methods 215-228 HPLC method development follows the series of steps summarized below. Information on sample, objective of separation. Need for special HPLC procedure, sample pretreatment etc. Choice of detector and detector settings. Choosing LC method, preliminary run, estimation of best separation conditions. Optimization of separation conditions. Check for problems or requirement for special procedure. a) Recovery of purified material   Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚   b) Quantitative calibration  Ã‚  Ã‚  Ã‚  Ã‚   c) Qualitative method Validate method for routine laboratory use. A good method development strategy should require only as many experimental runs as are necessary to achieve the desired final result. Finally, method development should be simple as possible, yet it should allow the use of sophisticated tools such as computer modeling if these are available. Before the beginning of method development, it is necessary to review what is known about the sample in order to define the goals of separation. The kinds of sample related information that can be important are summarized in Table-7.1. Table-8.1 Important information concerning sample composition and properties Number   of compounds present in the sample Chemical structures of components Molecular weights of compounds PKa values of compounds UV spectra of compounds Concentration range of various compounds in samples of interest Sample solubility   Ã‚   The chemical composition of the sample can provide valuable clues for the best choice of initial conditions for an HPLC separation. Objectives of separation The objectives of HPLC separation need to be specified clearly include. The use of HPLC to isolate purified sample components for spectral identification or quantitative analysis. It may be necessary to separate all degradants or impurities from a product for reliable content assay. In quantitative analysis, the required levels of accuracy and precision should be known (a precision of  ± 1 to 2% is usually achievable). Whether a single HPLC procedure is sufficient for raw material or one or more formulations and / or different procedures are desired for the analysis of formulations? When the number of samples for analysis at one time is greater than 10, a run time of less than 20 min. will be oftenly important. Knowledge on the desired HPLC equipment, experience and academic training the operators have. Sample pretreatment and detection Samples for analysis come in various forms such as: Solutions ready for injections. Solutions that require dilution, buffering, addition of an internal standard or other volumetric manipulation. Solids that must first be dissolved or extracted. Samples that require pretreatment to remove interference and/or protect the column or equipment from damage. Most samples for HPLC analysis require weighing and / or volumetric dilution before injection. Best results are often obtained when the composition of the sample solvent is close to that of the mobile phase since this minimizes baseline upset and other problems. Some samples require a partial separation ( pretreatment) prior to HPLC, because of need to remove interference, concentrate sample analytes or eliminate â€Å"column killer†. In many cases the development of an adequate sample pretreatment can be challenging than achieving a good HPLC separation. The detector selected should sense all sample components of interest. Variable-wavelength ultraviolet (UV) detectors normally are the first choice, because of their convenience and applicability for most samples. For this reason information on the UV spectra can be an important aid for method development. When the UV response of the sample is inadequate, other detectors are available (flourescence, electrochemical, PDA etc.) or the sample can be derivatized for enhanced detection. Developing the method for the separation Selecting an HPLC method and initial conditions If HPLC is chosen for the separation, the next step is to classify the sample as regular or special. Regular samples means typical mixtures of small molecules (    Table-8.2 Handling of special sample Sample Requirements Inorganic ions Detection is primary problems; use ion chromatography Isomers Some isomers can be separated by reversed-phase HPLC and are then classified as regular samples; better separations of isomers are obtainable using either (1) normal-phase HPLC or (2) reversed-phase separations with cyclodextrin-silica columns. Enantiomers These compounds require â€Å"chiral† conditions for their separations. Biological Several factors make samples or this kind â€Å"special†; molecular conformation, polar functionality and a wide range of hydrophobicity. Macromolecules â€Å"Big† molecules require column packing with large pores  Ã‚  (>> 10-nm diameters); in addition, biological molecules require special conditions as noted above. Table-8.3 Preferred experimental conditions for the initial HPLC separation Separation variable Preferred initial choice Column Dimensions (length, ID) 15 x 0.46 cm Particle size 5 mma Stationary phase C8 or C18 Mobile phase Solvent A and B Buffer-acetonitrile % B 80-100%b Buffer (compound, pH, concentration) 25mM potassium phosphate 2.0 Additives (e.g., amine modifiers, ion pair reagents) Do not use initially Flow rate 1.5–2.0 ml/min Temperature 35-45 ºC Sample Size Volumed >25 mL Weightd B : Polar solvent  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚   a 3.5 mm particles are an alternative using a 7.5 cm column b For an initial isocratic run; an initial gradient run is preferred. c No buffer required for neutral samples; for pH d Smaller values required for smaller-volume columns (e.g., 7.50.46-cm, 3.5-mm column). Table-8.4 Physical properties of silica supports for some C 18 columns Column (mL/mL) Pore diameter (nm) Surface area (m2/g) Percent Porosity Hypersil ODS 12 170 57 LiChrosorb C18 10 355 71 Novapak C18 6 N/Aa N/Aa Nucleosil C18 10 350 69` Symmetry C18 10 335 66 Zorbax ODS 6 300 55 Zorbax Rx, SB, XDB 8 180 50 a N/A : Not available On the basis of the initial exploratory run isocratic or gradient elution can be selected as most suitable. If typical reversed-phase conditions provide insufficient sample retention, suggesting the use of either ion pair on normal phase HPLC. Alternatively, the sample may be strongly retained with 100% acetonitrile as mobile phase, suggesting the use of non-aqueous reversed-phase (NARP) chromatography or normal phase HPLC. Some characteristics of reversed-phase and other HPLC methods are summarized below. Table-8.5 Characteristics of primary HPLC methods Method / description/ columns Preferred method Reversed-phase HPLC Uses water – organic mobile phase Columns: C18 (ODS), C8, phenyl, trimethylsilyl (TMS), Cyano First choice for most samples, especially neutral or non-ionisable compounds that dissolve in water-organic mixtures Ion-pair HPLC Uses water-organic mobile phase a buffer to control pH and an ion pair reagent. Column : C18, C8, cyano. Acceptable choice for ionic or ionizable compounds, especially bases or cations. Normal phase HPLC Uses mixtures of organic solvents as mobile phase Columns: Cyano, diol, amino and silica. Good second choice when reversed-phase or ion-pair HPLC is ineffective, first choice for lipophilic samples that do not dissolve well in water-organic mixtures, first choice for mixtures of isomers and for preparative-scale HPLC (silica best) Getting started on method development One approach is to use an isocratic mobile phase of some average solvent strength (e.g., 50%) organic solvent. A better alternative is to use a very strong mobile phase with (80-100% B), then reduce %B as necessary. The initial separation with 100%B results in rapid elution of the entire sample, but few groups will separate. Decreasing solvent strength shows the rapid separation of all components with a much longer run time, with a broadening of later bands and reduced detection sensitivity. Improving the separation and repeatable separation Generally the chromatographers will consider several aspects of the separation, as summarized in Table-8.6. Table-8.6 Objectives of separation in HPLC method development Objectivesa Comment Resolution Precise and rugged quantitative analysis requires that Rs be greater than 1.5. Separation time Quantitation   Ã‚ £ 2% (1 SD) for assays;  £ 5% for less-demanding analysis;  £15% for trace analysis. Pressure Peak height Narrow peaks are desirable for large signal / noise ratios Solvent consumption   Minimum mobile-phase use per run is desirable. a Roughly in order of decreasing importance but may vary with analysis requirements. Separation or resolution is a primary requirement in quantitative HPLC. The resolution (Rs) value should be maximum (Rs>1.5) favours maximum precision. Resolution usually degrades during the life of the column and can vary from day to day with minor fluctuations in separation conditions. Therefore, values of Rs = 2 or greater should be the goal during method development for simple mixtures. Such resolution will favour both improved assay precision and greater method ruggedness. Some HPLC assays do not require base line separation of the compounds of interest (qualitative analysis). In such cases only enough separation of individual components is required to provide characteristic retention times for peak identification. The time required for a separation (run time = retention time for base band) should be as short as possible and the total time spent on method development is reasonable (runtimes 5 to 10 minutes are desirable). Conditions for the final HPLC method should be selected so that the operating pressure with a new column does not exceed 170 bar (2500 psi) and upper pressure limit below 2000 psi is desirable. There are two reasons for that pressure limit, despite the fact that most HPLC equipment can be operated at much higher pressures. First, during the life of a column, the back pressure may rise by a factor of as much as 2 due to the gradual plugging of the column by particular matter. Second, at lower pressures When dealing with more challenging samples or if the goals of separation are particularly stringent, a large number of method development runs may be required to achieve acceptable separation. Repeatable separation As the experimental runs described above are being carried out, it is important to confirm that each chromatogram can be repeated. When changing conditions (mobile phase, column, and temperature) between method development experiments, enough time must elapse for the column to come into equilibrium with a new mobile phase and temperature. Usually column equilibration is achieved after passage of 10 to 20 column volumes of the new mobile phase through the column. However, this should be confirmed by carrying out a repeat experiment under the same conditions. When constant retention times are observed in two such back-to-back repeat experiments ( ± 0.5% or better), it can be assumed that the column is equilibrated and the experiments are repeatable. Completing the HPLC method development The final procedure should meet all the objectives that were defined at the beginning of method development. The method should also be robust in routine operation and usable by all laboratories and personnel for which it is intended. Quantitation and method validation One of the strengths of HPLC is that is an excellent quantitative analytical technique. HPLC can be used for the quantitation of the primary or major component of a sample (including pure samples) for mixture of many compounds at intermediate concentrations and for the assessment of trace impurity concentrations in matrix. Method validation, according to the United States Pharmacopoeia (USP), is performed to ensure that an analytical methodology is accurate, specific, reproducible and rugged over the specified range that an analyte will be analysed. Method validation provides an assurance of reliability during normal use and is sometimes described as the process of providing documented evidence that the method does what it is intended to do. According to USP, the method validation involves eight steps as given below. Precision Accuracy Limit of detection Limit of quantitation Specificity Linearity and range Ruggedness Robustness Precision and accuracy: Already discussed in chapter-1. Linearity The linearity of the method is a measure of how well a calibration plot of response v/s concentration approximates a straight line, or how well the data fit to the linear equation. Y = aX + b Where ‘Y’ is the response, ‘X’ is the concentration, ‘a’ is the slope and ‘b’ is the intercept of a line fit to the data. Ideally, a linear relationship is preferred (b = 0) because it is more precise, easier for calculations and can be defined with fewer standards. Also, UV detector response for a dilute sample is expected to follow Beer’s law and be linear. Therefore, a linear calibration gives evidence that the system is performing properly throughout the concentration range of interest. Generally in HPLC, if we are using internal standard, then the linearity plot is to be drawn by taking concentration of the analyte on x-axis and the ratio of area under the curve (AUC) of analyte to AUC of internal standard (IS) on y-axis. The resulting plot slope, intercept and correlation coefficient provide the desired information on linearity. A linearity correlation coefficient above 0.999 is acceptable for most methods. Limit of detection (LOD) The limit of detection (LOD) is the smallest concentration that can be detected reliably. The LOD represents the concentration of analyte that would yield a signal-to-noise (S/N) ratio of 3. Limit of quantitation (LOQ) The LOQ is the concentration that can be quantitated reliably with a specified level of accuracy and precision. The LOQ represents the concentration of analyte that would yield a signal-to-noise ratio of 10. LOD and LOQ can be determined by using the following expressions. LOD  Ã‚  Ã‚   =  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚   3 X N / B LOQ  Ã‚  Ã‚   =  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚   10 X N / B Where N is the noise estimate, is the standard deviation of the peak area ratio of analyte to IS (5 injections) of the drugs. B is the slope of the corresponding calibration curve. The LOD and LOQ values determined during method validation are affected by the separation conditions, columns, reagents and especially instrumentation and data systems. Ruggedness Method ruggedness is defined as the reproducibility of results when the method is performed under actual use conditions. This includes different analysts, laboratories, columns, instruments, sources, chemicals, solvents etc. method ruggedness may not be known when a method is first developed, but insight is obtained during subsequent use of that method. Robustness The concept of robustness of an analytical procedure has been defined by the ICH as â€Å" a measure of its capacity to remain unaffected by small, but deliberate variations in method parameters†. The robustness of a method is the ability to remain unaffected by small changes in parameters such as pH of the mobile phase, temperature, percentage of organic solvent and buffer concentration etc. to determine robustness of the method experimental conditions were purposely altered and chromatographic characteristics were evaluated. To study the pH effect on the retention (K1) of the drug, buffer pH is to be changed by 0.2 units. At certain point, retention will increase at any pH above and below of the pH unit. The effect of temperature on the retention characteristics (K1) of the drug is to be studied by changing the temperature in steps 2 ºC from room temperature to 80 ºC and see the effect of temperature on the resolution and peak shape. Effect of percentage organic strength on retention is to be studied by varying the percentage of organic solvents like acetonitrile, methanol etc. from 0 to 2% while the other mobile phase contents are held constant and observe the K1. At certain point decreases in K1 observed with increase in the level of organic solvent. Effect of buffer concentration should be checked at three concentration levels i.e. 0.025 M, 0.05 M and 0.1 M and observe retention time and resolution. Stability To generate reproducible and reliable results, the samples, standards and reagents used for the HPLC method must be stable for a reasonable time (e.g., One day, one week, one month, depending on the need). For example, the analysis of even a single sample may require 10 or more chromatographic runs to determine system suitability, including standard concentrations to create a working analytical curve and duplicate or triplicate injections of the sample to be assayed. Therefore, a few hours of standard and sample solution stability can be required even for a short (10 min.) separation. When more than one sample is analyzed, automated, over night runs often are performed for better laboratory efficiency. Typically, 24 hours stability is desired for all solutions and reagents that need to be prepared for each analysis. Mobile phases should be chosen to avoid stability problems, especially the use of amine additives or specific solvents. For example, mobile phase containing THF (tetra hydrofuran) are known to be susceptible to oxidation, therefore, the mobile phase should be prepared daily with fresh THF. Some buffered mobile phases cause problems for example, phosphate and acetate provide good media for microbial growth. Sodium oxide (0.1%) is often added to the mobile phase buffer to inhibit such growth, adding more than 5% of organic solvent is also effective. Long term column stability is critical for method ruggedness. Even the best HPLC column will eventually degrade and lose its initial performance, often as a function of the number of samples injected. System suitability System suitability experiments can be defined as tests to ensure that the method can generate results of acceptable accuracy and precision. The requirements for system suitability are usually developed after method development and validation have been completed. The criteria selected will be based on the actual performance of the method as determined during its validation. For example, if sample retention times forms part of the system suitability criteria, their variation (SD) during validation can be determined, system suitability might then require that retention times fall within a  ±3 SD range during routine performance of the method. The USP (2000) defines parameters that can be used to determine system suitability prior to analysis. These parameters include plate number (N), tailing factor, k and / or a, resolution (Rs) and relative standard deviation (RSD) of peak height or peak area for respective injections. The RSD of peak height or area of five injections of standard solution is normally accepted as one of the standard criteria. For an assay method of a major component, the RSD should typically be less than 1% for these five respective injections. The plate number and / or tailing factor are used if the run contains only one peak. For chromatographic separations with more than one peak, such as an internal standard assay or an impurity method, expected to contain many peaks, some measure of separations such as Rs is recommended. Reproducibility of tR or k value for a specific compound also defines system performance. The column performance can be defined in terms of column plate number ‘N’ is defined by N = 5.54 (tR / W ½)2 Where ‘tR’ is the retention time of the peak and ‘W ½Ã¢â‚¬â„¢ is the width of the peak at half peak height. The resolution of two adjacent peaks can be calculated by using the formula Rs = 1.18 (t2-t1) / W0.5.1 +W0.5.2 Where ‘t1’ and ‘t2’ are retention times of the adjacent peaks and W0.5.1 and W0.5.2 are the width of the peaks at half height. Rs = 2.0 or greater is a desirable target for method development. The retention factor k is given by the equation. k = (tR – t0) / t0 where ‘tR’ is the band retention time and t0 is the column dead time. The peak symmetry can be represented in terms of peak asymmetry factor and peak tailing factor, which can be calculated by using the following formula. Peak asymmetry factor = B /A Where ‘B’ is the distance at 50% peak height between leading edge to the perpendicular drawn from the peak maxima and ‘A’ is the width of the peak at half height. According to USP (2000) peak tailing factor can be calculated by using the formula T = W0.05 / 2f Where â€Å"W0.05† is the width of the peak at 5% height and â€Å"f† is the distance from the peak maximum to the leading edge of the peak, the distance being measured at a point 50% of the peak height from the base line. High Performance Liquid Chromatography (HPLC) 214 High Performance Liquid Chromatography (HPLC) 214 Introduction High performance liquid chromatography 214 is the most widely used of all of the analytical separation techniques. The reasons for the popularity of the method is its sensitivity, ready adaptability to accurate quantitative determinations, suitability for separating non-volatile species or thermally fragile ones, wide spread applicability to substance that are of prime interest to industry, many fields of science and the public. The applications of chromatography have grown explosively in the last fifty years owing not only to the development of several new types of chromatographic techniques but also to the growing need by scientist for better methods for characterizing complex mixtures. General methodology for the development of new HPLC methods 215-228 HPLC method development follows the series of steps summarized below. Information on sample, objective of separation. Need for special HPLC procedure, sample pretreatment etc. Choice of detector and detector settings. Choosing LC method, preliminary run, estimation of best separation conditions. Optimization of separation conditions. Check for problems or requirement for special procedure. a) Recovery of purified material   Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚   b) Quantitative calibration  Ã‚  Ã‚  Ã‚  Ã‚   c) Qualitative method Validate method for routine laboratory use. A good method development strategy should require only as many experimental runs as are necessary to achieve the desired final result. Finally, method development should be simple as possible, yet it should allow the use of sophisticated tools such as computer modeling if these are available. Before the beginning of method development, it is necessary to review what is known about the sample in order to define the goals of separation. The kinds of sample related information that can be important are summarized in Table-7.1. Table-8.1 Important information concerning sample composition and properties Number   of compounds present in the sample Chemical structures of components Molecular weights of compounds PKa values of compounds UV spectra of compounds Concentration range of various compounds in samples of interest Sample solubility   Ã‚   The chemical composition of the sample can provide valuable clues for the best choice of initial conditions for an HPLC separation. Objectives of separation The objectives of HPLC separation need to be specified clearly include. The use of HPLC to isolate purified sample components for spectral identification or quantitative analysis. It may be necessary to separate all degradants or impurities from a product for reliable content assay. In quantitative analysis, the required levels of accuracy and precision should be known (a precision of  ± 1 to 2% is usually achievable). Whether a single HPLC procedure is sufficient for raw material or one or more formulations and / or different procedures are desired for the analysis of formulations? When the number of samples for analysis at one time is greater than 10, a run time of less than 20 min. will be oftenly important. Knowledge on the desired HPLC equipment, experience and academic training the operators have. Sample pretreatment and detection Samples for analysis come in various forms such as: Solutions ready for injections. Solutions that require dilution, buffering, addition of an internal standard or other volumetric manipulation. Solids that must first be dissolved or extracted. Samples that require pretreatment to remove interference and/or protect the column or equipment from damage. Most samples for HPLC analysis require weighing and / or volumetric dilution before injection. Best results are often obtained when the composition of the sample solvent is close to that of the mobile phase since this minimizes baseline upset and other problems. Some samples require a partial separation ( pretreatment) prior to HPLC, because of need to remove interference, concentrate sample analytes or eliminate â€Å"column killer†. In many cases the development of an adequate sample pretreatment can be challenging than achieving a good HPLC separation. The detector selected should sense all sample components of interest. Variable-wavelength ultraviolet (UV) detectors normally are the first choice, because of their convenience and applicability for most samples. For this reason information on the UV spectra can be an important aid for method development. When the UV response of the sample is inadequate, other detectors are available (flourescence, electrochemical, PDA etc.) or the sample can be derivatized for enhanced detection. Developing the method for the separation Selecting an HPLC method and initial conditions If HPLC is chosen for the separation, the next step is to classify the sample as regular or special. Regular samples means typical mixtures of small molecules (    Table-8.2 Handling of special sample Sample Requirements Inorganic ions Detection is primary problems; use ion chromatography Isomers Some isomers can be separated by reversed-phase HPLC and are then classified as regular samples; better separations of isomers are obtainable using either (1) normal-phase HPLC or (2) reversed-phase separations with cyclodextrin-silica columns. Enantiomers These compounds require â€Å"chiral† conditions for their separations. Biological Several factors make samples or this kind â€Å"special†; molecular conformation, polar functionality and a wide range of hydrophobicity. Macromolecules â€Å"Big† molecules require column packing with large pores  Ã‚  (>> 10-nm diameters); in addition, biological molecules require special conditions as noted above. Table-8.3 Preferred experimental conditions for the initial HPLC separation Separation variable Preferred initial choice Column Dimensions (length, ID) 15 x 0.46 cm Particle size 5 mma Stationary phase C8 or C18 Mobile phase Solvent A and B Buffer-acetonitrile % B 80-100%b Buffer (compound, pH, concentration) 25mM potassium phosphate 2.0 Additives (e.g., amine modifiers, ion pair reagents) Do not use initially Flow rate 1.5–2.0 ml/min Temperature 35-45 ºC Sample Size Volumed >25 mL Weightd B : Polar solvent  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚   a 3.5 mm particles are an alternative using a 7.5 cm column b For an initial isocratic run; an initial gradient run is preferred. c No buffer required for neutral samples; for pH d Smaller values required for smaller-volume columns (e.g., 7.50.46-cm, 3.5-mm column). Table-8.4 Physical properties of silica supports for some C 18 columns Column (mL/mL) Pore diameter (nm) Surface area (m2/g) Percent Porosity Hypersil ODS 12 170 57 LiChrosorb C18 10 355 71 Novapak C18 6 N/Aa N/Aa Nucleosil C18 10 350 69` Symmetry C18 10 335 66 Zorbax ODS 6 300 55 Zorbax Rx, SB, XDB 8 180 50 a N/A : Not available On the basis of the initial exploratory run isocratic or gradient elution can be selected as most suitable. If typical reversed-phase conditions provide insufficient sample retention, suggesting the use of either ion pair on normal phase HPLC. Alternatively, the sample may be strongly retained with 100% acetonitrile as mobile phase, suggesting the use of non-aqueous reversed-phase (NARP) chromatography or normal phase HPLC. Some characteristics of reversed-phase and other HPLC methods are summarized below. Table-8.5 Characteristics of primary HPLC methods Method / description/ columns Preferred method Reversed-phase HPLC Uses water – organic mobile phase Columns: C18 (ODS), C8, phenyl, trimethylsilyl (TMS), Cyano First choice for most samples, especially neutral or non-ionisable compounds that dissolve in water-organic mixtures Ion-pair HPLC Uses water-organic mobile phase a buffer to control pH and an ion pair reagent. Column : C18, C8, cyano. Acceptable choice for ionic or ionizable compounds, especially bases or cations. Normal phase HPLC Uses mixtures of organic solvents as mobile phase Columns: Cyano, diol, amino and silica. Good second choice when reversed-phase or ion-pair HPLC is ineffective, first choice for lipophilic samples that do not dissolve well in water-organic mixtures, first choice for mixtures of isomers and for preparative-scale HPLC (silica best) Getting started on method development One approach is to use an isocratic mobile phase of some average solvent strength (e.g., 50%) organic solvent. A better alternative is to use a very strong mobile phase with (80-100% B), then reduce %B as necessary. The initial separation with 100%B results in rapid elution of the entire sample, but few groups will separate. Decreasing solvent strength shows the rapid separation of all components with a much longer run time, with a broadening of later bands and reduced detection sensitivity. Improving the separation and repeatable separation Generally the chromatographers will consider several aspects of the separation, as summarized in Table-8.6. Table-8.6 Objectives of separation in HPLC method development Objectivesa Comment Resolution Precise and rugged quantitative analysis requires that Rs be greater than 1.5. Separation time Quantitation   Ã‚ £ 2% (1 SD) for assays;  £ 5% for less-demanding analysis;  £15% for trace analysis. Pressure Peak height Narrow peaks are desirable for large signal / noise ratios Solvent consumption   Minimum mobile-phase use per run is desirable. a Roughly in order of decreasing importance but may vary with analysis requirements. Separation or resolution is a primary requirement in quantitative HPLC. The resolution (Rs) value should be maximum (Rs>1.5) favours maximum precision. Resolution usually degrades during the life of the column and can vary from day to day with minor fluctuations in separation conditions. Therefore, values of Rs = 2 or greater should be the goal during method development for simple mixtures. Such resolution will favour both improved assay precision and greater method ruggedness. Some HPLC assays do not require base line separation of the compounds of interest (qualitative analysis). In such cases only enough separation of individual components is required to provide characteristic retention times for peak identification. The time required for a separation (run time = retention time for base band) should be as short as possible and the total time spent on method development is reasonable (runtimes 5 to 10 minutes are desirable). Conditions for the final HPLC method should be selected so that the operating pressure with a new column does not exceed 170 bar (2500 psi) and upper pressure limit below 2000 psi is desirable. There are two reasons for that pressure limit, despite the fact that most HPLC equipment can be operated at much higher pressures. First, during the life of a column, the back pressure may rise by a factor of as much as 2 due to the gradual plugging of the column by particular matter. Second, at lower pressures When dealing with more challenging samples or if the goals of separation are particularly stringent, a large number of method development runs may be required to achieve acceptable separation. Repeatable separation As the experimental runs described above are being carried out, it is important to confirm that each chromatogram can be repeated. When changing conditions (mobile phase, column, and temperature) between method development experiments, enough time must elapse for the column to come into equilibrium with a new mobile phase and temperature. Usually column equilibration is achieved after passage of 10 to 20 column volumes of the new mobile phase through the column. However, this should be confirmed by carrying out a repeat experiment under the same conditions. When constant retention times are observed in two such back-to-back repeat experiments ( ± 0.5% or better), it can be assumed that the column is equilibrated and the experiments are repeatable. Completing the HPLC method development The final procedure should meet all the objectives that were defined at the beginning of method development. The method should also be robust in routine operation and usable by all laboratories and personnel for which it is intended. Quantitation and method validation One of the strengths of HPLC is that is an excellent quantitative analytical technique. HPLC can be used for the quantitation of the primary or major component of a sample (including pure samples) for mixture of many compounds at intermediate concentrations and for the assessment of trace impurity concentrations in matrix. Method validation, according to the United States Pharmacopoeia (USP), is performed to ensure that an analytical methodology is accurate, specific, reproducible and rugged over the specified range that an analyte will be analysed. Method validation provides an assurance of reliability during normal use and is sometimes described as the process of providing documented evidence that the method does what it is intended to do. According to USP, the method validation involves eight steps as given below. Precision Accuracy Limit of detection Limit of quantitation Specificity Linearity and range Ruggedness Robustness Precision and accuracy: Already discussed in chapter-1. Linearity The linearity of the method is a measure of how well a calibration plot of response v/s concentration approximates a straight line, or how well the data fit to the linear equation. Y = aX + b Where ‘Y’ is the response, ‘X’ is the concentration, ‘a’ is the slope and ‘b’ is the intercept of a line fit to the data. Ideally, a linear relationship is preferred (b = 0) because it is more precise, easier for calculations and can be defined with fewer standards. Also, UV detector response for a dilute sample is expected to follow Beer’s law and be linear. Therefore, a linear calibration gives evidence that the system is performing properly throughout the concentration range of interest. Generally in HPLC, if we are using internal standard, then the linearity plot is to be drawn by taking concentration of the analyte on x-axis and the ratio of area under the curve (AUC) of analyte to AUC of internal standard (IS) on y-axis. The resulting plot slope, intercept and correlation coefficient provide the desired information on linearity. A linearity correlation coefficient above 0.999 is acceptable for most methods. Limit of detection (LOD) The limit of detection (LOD) is the smallest concentration that can be detected reliably. The LOD represents the concentration of analyte that would yield a signal-to-noise (S/N) ratio of 3. Limit of quantitation (LOQ) The LOQ is the concentration that can be quantitated reliably with a specified level of accuracy and precision. The LOQ represents the concentration of analyte that would yield a signal-to-noise ratio of 10. LOD and LOQ can be determined by using the following expressions. LOD  Ã‚  Ã‚   =  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚   3 X N / B LOQ  Ã‚  Ã‚   =  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚   10 X N / B Where N is the noise estimate, is the standard deviation of the peak area ratio of analyte to IS (5 injections) of the drugs. B is the slope of the corresponding calibration curve. The LOD and LOQ values determined during method validation are affected by the separation conditions, columns, reagents and especially instrumentation and data systems. Ruggedness Method ruggedness is defined as the reproducibility of results when the method is performed under actual use conditions. This includes different analysts, laboratories, columns, instruments, sources, chemicals, solvents etc. method ruggedness may not be known when a method is first developed, but insight is obtained during subsequent use of that method. Robustness The concept of robustness of an analytical procedure has been defined by the ICH as â€Å" a measure of its capacity to remain unaffected by small, but deliberate variations in method parameters†. The robustness of a method is the ability to remain unaffected by small changes in parameters such as pH of the mobile phase, temperature, percentage of organic solvent and buffer concentration etc. to determine robustness of the method experimental conditions were purposely altered and chromatographic characteristics were evaluated. To study the pH effect on the retention (K1) of the drug, buffer pH is to be changed by 0.2 units. At certain point, retention will increase at any pH above and below of the pH unit. The effect of temperature on the retention characteristics (K1) of the drug is to be studied by changing the temperature in steps 2 ºC from room temperature to 80 ºC and see the effect of temperature on the resolution and peak shape. Effect of percentage organic strength on retention is to be studied by varying the percentage of organic solvents like acetonitrile, methanol etc. from 0 to 2% while the other mobile phase contents are held constant and observe the K1. At certain point decreases in K1 observed with increase in the level of organic solvent. Effect of buffer concentration should be checked at three concentration levels i.e. 0.025 M, 0.05 M and 0.1 M and observe retention time and resolution. Stability To generate reproducible and reliable results, the samples, standards and reagents used for the HPLC method must be stable for a reasonable time (e.g., One day, one week, one month, depending on the need). For example, the analysis of even a single sample may require 10 or more chromatographic runs to determine system suitability, including standard concentrations to create a working analytical curve and duplicate or triplicate injections of the sample to be assayed. Therefore, a few hours of standard and sample solution stability can be required even for a short (10 min.) separation. When more than one sample is analyzed, automated, over night runs often are performed for better laboratory efficiency. Typically, 24 hours stability is desired for all solutions and reagents that need to be prepared for each analysis. Mobile phases should be chosen to avoid stability problems, especially the use of amine additives or specific solvents. For example, mobile phase containing THF (tetra hydrofuran) are known to be susceptible to oxidation, therefore, the mobile phase should be prepared daily with fresh THF. Some buffered mobile phases cause problems for example, phosphate and acetate provide good media for microbial growth. Sodium oxide (0.1%) is often added to the mobile phase buffer to inhibit such growth, adding more than 5% of organic solvent is also effective. Long term column stability is critical for method ruggedness. Even the best HPLC column will eventually degrade and lose its initial performance, often as a function of the number of samples injected. System suitability System suitability experiments can be defined as tests to ensure that the method can generate results of acceptable accuracy and precision. The requirements for system suitability are usually developed after method development and validation have been completed. The criteria selected will be based on the actual performance of the method as determined during its validation. For example, if sample retention times forms part of the system suitability criteria, their variation (SD) during validation can be determined, system suitability might then require that retention times fall within a  ±3 SD range during routine performance of the method. The USP (2000) defines parameters that can be used to determine system suitability prior to analysis. These parameters include plate number (N), tailing factor, k and / or a, resolution (Rs) and relative standard deviation (RSD) of peak height or peak area for respective injections. The RSD of peak height or area of five injections of standard solution is normally accepted as one of the standard criteria. For an assay method of a major component, the RSD should typically be less than 1% for these five respective injections. The plate number and / or tailing factor are used if the run contains only one peak. For chromatographic separations with more than one peak, such as an internal standard assay or an impurity method, expected to contain many peaks, some measure of separations such as Rs is recommended. Reproducibility of tR or k value for a specific compound also defines system performance. The column performance can be defined in terms of column plate number ‘N’ is defined by N = 5.54 (tR / W ½)2 Where ‘tR’ is the retention time of the peak and ‘W ½Ã¢â‚¬â„¢ is the width of the peak at half peak height. The resolution of two adjacent peaks can be calculated by using the formula Rs = 1.18 (t2-t1) / W0.5.1 +W0.5.2 Where ‘t1’ and ‘t2’ are retention times of the adjacent peaks and W0.5.1 and W0.5.2 are the width of the peaks at half height. Rs = 2.0 or greater is a desirable target for method development. The retention factor k is given by the equation. k = (tR – t0) / t0 where ‘tR’ is the band retention time and t0 is the column dead time. The peak symmetry can be represented in terms of peak asymmetry factor and peak tailing factor, which can be calculated by using the following formula. Peak asymmetry factor = B /A Where ‘B’ is the distance at 50% peak height between leading edge to the perpendicular drawn from the peak maxima and ‘A’ is the width of the peak at half height. According to USP (2000) peak tailing factor can be calculated by using the formula T = W0.05 / 2f Where â€Å"W0.05† is the width of the peak at 5% height and â€Å"f† is the distance from the peak maximum to the leading edge of the peak, the distance being measured at a point 50% of the peak height from the base line.

Cluniac Monasticism Essay -- History

Cluniac Monasticism Assess the strengths and weaknesses of Cluniac monasticism between the tenth and twelfth centuries. The nature of Cluny lay in the circumstances of it’s foundation. It was endowed with a measure of independence by it’s founder, Duke William, allowing the monks to elect their own abbot, placing the abbey directly under the guardianship of St Peter and the Apostolic See. As a house dedicated to reviving strict Benedictine observance Cluny was not unique, but it was this indepencence, the succesion of talented abbots and it’s organisation set up by Abbot Berno that laid the foundations of the abbey’s later greatness. The independence granted Cluny in it’s foundation charter was esssential in the development of Cluny free from the interference of lay magnates and local bishops. It’s direct dependence on Rome was not initially of great importence; other foundations had beemn bequeathed to the apostles before. However, this was an important foundation upon which later abbots were to build. By seeking papal approval for Cluniac reforms the abbots forged a valuble direct link to the papacy, whilst gain officail public regognition and endorsement of the Cluniac regieme. In obtaining the right to accept monks from other orders in 931 Odo had confirmed the righht of the Cluniacs to reform others houses, while Cluny gained freedom from the local bishops under Abbot Odilo in 998. it was ‘the subsequent growth, under far straiter papal oversight, of Cluny’s exemption from episcopal control in spiritual matters, that did most to consolidate Cluny’s subject houses under itâ⠂¬â„¢s own central authority’ . This meant Cluny was immune to challenges to it’s authority from both without and within the church. Cluny became of particular note to sucessive Popes, with it’s reputation for reform, and the papacy continued to support the cluniacs, with Pope John XIX giving Cluny’s monks complete freedom from interference whereever they were in 1024. However, this would have been nothing without the exemplary spiritual life that was seen to exist at Cluny. The continual use of vocal prayer was popular with the laity, with many wishing to be included in the prayers of the monks. The personal qualities of the abbots were also much admired, as was the way of life practised at Cluny. The cluniac model of benedictine obervence was seen by many by the time of Abb... ... riches of success. The Cluniacs were criticised by those who favoured a more eremetic style of monasticism, and the stricter Cistercians. They were also criticised by laymen and other factions within the church. As an order, their popularity was on the wane by the twelfth century. The main strenghts of cluniac monasticism lay in its independence, it’s early spiritual energy, it’s observence of the benedictine rule and it’s sainly abbots. These provided very well for Cluny for a significant period, but over-growth of the order led to a slackening in observence of the Rule, and with the disasterous abbacy of Pons and a basic shift in religious opinion these strenghts became weaknesses that Bibliography Evans, Monastic Life at Cluny 910-1157 (Oxford University Press, 1931) Ed. Hunt, Cluniac Monasticsm in the central middle ages, (Macmillan, 1971) Lynch, The Medeval Church, (Longman, 1992) Cowdrey, The Cluniacs and the Gregorian Reform, (Oxford, 1970) Lawrence, Medeval Monasticism, (Longman, 1989) Southern, Western Society and the Church in the Middle Ages, (Pelican, 1970) Ed. Holmes, The Oxford Illustrated History of Medeval Europe, (Oxford, 2001)